Active oxygen induced protein ubiquitination in Chlamydomonas

FEBS Letters

Muto, Shoshi¹  Shimogawara, Kousuke¹ 

[1] Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan

关键词: Active oxygen stress;    Stress response;    Methylviologen;    Ubiquitin;    Chlamydomonas reinhardtil;   

DOI  :  10.1016/0014-5793(91)80130-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When methylviologen-treated Chlamydomonas cells were exposed to light, the amount of ubiquitinated proteins with molecular masses of 28- and 31-kDa were drastically changed, i.e. the former increased within 20 min illumination, while the latter decreased. Since these changes are completely dependent on illumination and suppressed by adding 3-(3,4-dichlorophenyl)-1, 1-dimethylurea, it was concluded that these changes were caused by active oxygen stress. Treating cells with hydrogen peroxide did not cause such changes of ubiquitination, suggesting that the molecular species of active oxygen is a superoxide anion.

【 授权许可】

Unknown