Mitochondria catalyze the reduction of NAD by reduced methylviologen

FEBS Letters

Nagata, Shinichi¹  Günther, Helmut¹  Simon, Helmut¹  Bader, Johann¹ 

[1] Institute for Organic Chemistry, Technical University Munich, D-8046 Garching, FRG

关键词: Mitochondria;    Methylviologen;    NADH formation;    Stereospecificity;    NADH deuteration;   

DOI  :  10.1016/0014-5793(87)81299-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondria from beef heart and yeast catalyze the reduction of NAD to NADH at the expense of reduced methylviologen (MV⁺). Based on protein the specific activity of mitochondria for this reaction is about 10–20-times higher than the consumption of oxygen in the presence of succinate or NADH. In ²H₂O buffer (4S)-[4-²H]NADH is formed in high enantiomeric excess if the reduced methylviologen is electrochemically regenerated.

【 授权许可】

Unknown