K+ channel expression in primary cell cultures mediated by vaccinia virus

FEBS Letters

Gouin, Annie¹  Lester, Henry A.¹  Davidson, Norman¹  Karschin, Andreas¹  Alyar, Jayashree¹ 

[1] Division of Biology 154-29 California Institute of Technology, Pasadena, CA 91125, USA

关键词: Heterologous expression;    Shaker;    Voltage-gated;    Ion channel;    Excitability;   

DOI  :  10.1016/0014-5793(91)80123-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recombinant vaccinia virus (VV) was used to express functional Drosophila Shaker H4 K⁺ channels in primary cell cultures from rat heart (atrial and ventricular myocytes, fibroblasts), autonomic ganglia (SCG neurons) and CNS (hippocampal neurons, cerebral astroglia). In most cells the expressed currents possessed the typical characteristics of the native Drosophila muscle A currents; a few cells showed evidence of hetero-oligomers with new properties. The maximum current density corresponded to a channel density of 2–3/μm³. Voltage recordings in heart cells showed altered action potential waveforms after successful infection. VV vectors thus are useful for studying altered excitability and cell-specific processing of ion channel proteins.

【 授权许可】

Unknown