Thimerosal causes calcium oscillations and sensitizes calcium‐induced calcium release in unfertilized hamster eggs

FEBS Letters

Swann, Karl¹ 

[1]MRC Experimental Embryology and Teratology Unit. St. George's Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK

关键词: Calcium ion release;    Hamster egg;    Calcium Activated potassium conductance;    Sulfhydryl reagent;    HR;    hyperpolarizing membrane potential response;    CICR;    calcium-induced-calcium-released;    Ca2+;    calcium ions;    [Ca2+]1;    intracellular free calcium concentration;    NEM;    n-ethylmaleimide;    InsP3;    inositol 1;    4;    5-triphosphate;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(91)80110-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calcium-induced-calcium-release (CICR) was assayed in unfertilized golden hamster eggs by injecting Ca²⁺ and monitoring Ca²⁺-dependent hyper-polarizing responses (HRs) and Ca²⁺-sensitive fluo-3 fluorescence. Incubating eggs in the sulfhydryl reagent thimerosal caused [Ca²⁺]₁ oscillations as monitored by Ca²⁺-dependent HRs and decreased approximately 10-fold the Ca²⁺ injection current required to generate an HR and cause a large intracellular Ca²⁺ increase. Thimerosal also enhanced the sensitivity of eggs to Ca²⁺ injection in a calcium-free medium. The effects of thimerosal on CICR were prevented by dithiothreitol and were not mimicked by injecting inositol 1,4,5-triphosphate. The data suggest that thimerosal may be an alternative agent for studying CICR in caffeine-insensitive cells.

【 授权许可】

Unknown