FEBS Letters | |
Thimerosal causes calcium oscillations and sensitizes calcium‐induced calcium release in unfertilized hamster eggs | |
Swann, Karl1  | |
[1] MRC Experimental Embryology and Teratology Unit. St. George's Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK | |
关键词: Calcium ion release; Hamster egg; Calcium Activated potassium conductance; Sulfhydryl reagent; HR; hyperpolarizing membrane potential response; CICR; calcium-induced-calcium-released; Ca2+; calcium ions; [Ca2+]1; intracellular free calcium concentration; NEM; n-ethylmaleimide; InsP3; inositol 1; 4; 5-triphosphate; DTT; dithiothreitol; | |
DOI : 10.1016/0014-5793(91)80110-O | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Calcium-induced-calcium-release (CICR) was assayed in unfertilized golden hamster eggs by injecting Ca2+ and monitoring Ca2+-dependent hyper-polarizing responses (HRs) and Ca2+-sensitive fluo-3 fluorescence. Incubating eggs in the sulfhydryl reagent thimerosal caused [Ca2+]1 oscillations as monitored by Ca2+-dependent HRs and decreased approximately 10-fold the Ca2+ injection current required to generate an HR and cause a large intracellular Ca2+ increase. Thimerosal also enhanced the sensitivity of eggs to Ca2+ injection in a calcium-free medium. The effects of thimerosal on CICR were prevented by dithiothreitol and were not mimicked by injecting inositol 1,4,5-triphosphate. The data suggest that thimerosal may be an alternative agent for studying CICR in caffeine-insensitive cells.
【 授权许可】
Unknown
【 预 览 】
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