Use of electrospray mass spectrometry to directly observe an acyl enzyme intermediate in β‐lactamase catalysis

FEBS Letters

Waley, Stephen G.¹  Schofield, Christopher J.²  Aplin, Robin T.²  Baldwin, Jack E.² 

[1] The Laboratory of Molecular Biophysics and the Oxford Centre for Molecular Sciences, South Parks Road, Oxford, 0X1 3QY, UK;The Dyson Perrins Laboratory, South Parks Road, Oxford, 0X1 3QY, UK

关键词: β-Lactamase;    Electrospray mass spectrometry;    Inhibition;    Acyl enzyme complex;   

DOI  :  10.1016/0014-5793(90)80847-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electrospray mass spectrometry was used to directly observe intact acyl enzyme complexes formed between a class C β-lactamase (from Enterobacter cloacae P99) and four poor substrates/inhibitors. In each case the molecular weight difference between the unreacted and the reacted β-lactamase was consistent with the formation of an acyl enzyme.

【 授权许可】

Unknown