Formation of tritium‐labeled polysialylated gangliosides in the cytosol of rat cerebellar granule cells in culture following administration of [3H]GM1 ganglioside

FEBS Letters

Valsecchi, Manuela¹  Chigorno, Vanna¹  Sonnino, Sandro¹  Tettamanti, Guido¹ 

[1] Study Center for the Functional Biochemistry of Brain Lipids, Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Via Saldini 50, 20133 Milan, Italy

关键词: Ganglioside;    Cytosol;    Biosynthesis;    Transport;   

DOI  :  10.1016/0014-5793(90)80834-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GM1 ganglioside tritium-labeled at C-3 of sphingosine has been administered to rat cerebellar granule cells. Tritiated polysialylated gangliosides were observed in the cytosol of the cells, where they resulted in a higher amount after a short period of chase. This, together with the data showing an increase of the tritiated polysialylated gangliosides in the total particulate fraction in parallel to the prolonging of the chase period, suggests that cytosolic gangliosides could be a way of transporting neosynthesized gangliosides from the Golgi apparatus to the plasma membranes.

【 授权许可】

Unknown