Studies on the primary sequence requirements for PKC‐α, ‐β1 and ‐γ peptide substrates

FEBS Letters

Woodgett, James R.¹  Parker, Peter J.¹  Nguyen, Oanh¹  Marais, Richard M.¹ 

[1] Ludwig Institute for Cancer Research, Courtauld Building, 91 Riding House Street, London W1P 8BT, UK

关键词: Protein kinase C;    Kinase specificity;    Synthetic peptide;   

DOI  :  10.1016/0014-5793(90)80831-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The substrate specificity of purified PKC-α, -β and -γ has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-α and -β are indistinguishable in their phosphorylation of these peptides, PKC-γ shows a distinct specificity profile for these synthetic substrates.

【 授权许可】

Unknown