Lethal neurotoxicity in mice of the basic domains of HIV and SIV Rev proteins Study of these regions by circular dichroism

FEBS Letters

Van Rietschoten, Jurphaas²  Darbon, Hervé¹  Mabrouk, Kamel²  Rochat, Hervé²  Vives, Eric²  Sabatier, Jean-Marc² 

[1] LCCMB, CNRS URA 1296, Facultè de Mèdecine Secteur Nord, Bd P. Dramard, 13326-Marseille Cédex 15, France;Laboratoire de Biochemie, CNRS URA 1455, Facultè de Mèdecine Secteur Nord, Bd P. Dramard, 13326-Marseille Cèdex 15, France

关键词: Retrovirus;    Trans-activator;    Basic domain;    Synthetic peptide;    Neurotoxicity;    Circular dichroism;   

DOI  :  10.1016/0014-5793(91)80898-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently reported a basic domain-mediated neurotoxic activity of HIV-1 Tat [1991, J. Virol. 65, 961–965]. Here we have tested the neurotoxicity in vivo of several Rev-related synthetic peptides and found that only those mimicking the basic regions of Rev from HIV-1, HIV-2 and SIV were lethal to mice. In contrast, the homologous domain of HTLV-1 Rex was found to be inactive for lethal activity. Analysis of the tropism of these peptides for phospholipids has demonstrated a direct interaction of the basic domain-containing peptides, except Rex, with acidic — but not neutral — phospholipids. As determined by circular dichroism, a possible correlation between the conformation of the basic regions and the toxicity is discussed.

【 授权许可】

Unknown