Interaction of puromycin with acceptor site of human placenta 80 S ribosomes

FEBS Letters

Karpova, G.G.¹  Fedorova, O.S.¹  Graifer, D.M.¹ 

[1] Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: Puromycin;    Ribosome;    Human placenta;    Rate constant;    Affinity;   

DOI  :  10.1016/0014-5793(90)80795-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complex N-AcPhe-tRNA^Phe · poly(U) · 80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N-acetylphenylalanyl-puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 ± 0.84) × 10⁴ M⁻¹ at 37°C.

【 授权许可】

Unknown