Activation of red cell Ca2+ ‐activated K+ channel by Ca2+ involves a temperature‐dependent step

FEBS Letters

Peterajová, Elena¹  Varečka, Ĺudovít¹ 

[1] Mental Health Research Center of the Medical Bionics Institute, Psychiatric Hospital, 90218-Pezinok, Czechoslovakia

关键词: Red cell;    Vanadate;    Propronolol;    Ca2+-activated K+ channel;   

DOI  :  10.1016/0014-5793(90)80534-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found that vanadate-induced ⁴⁵Ca²⁺ uptake by red cells is maximal at 25°C. At this temperature, the Ca_i-induced increase of the K⁺ permeability (the Gardos effect) shows a lag (up to 8 min) which is not observed at 37°C. This cannot be explained by the lack of availability of Ca²⁺ for the Ca²⁺-activated K⁺ channel, and suggests that its activation by Ca²⁺ is mediated by a temperature-dependent mechanism which remains unknown so far. The lag is not observed when the Gárdos effect was initiated by propranolol. This shows that the putative temperature-dependent step is different from chloride transport.

【 授权许可】

Unknown