Effects of pressure on glucose transport in human erythrocytes

FEBS Letters

Lowe, A.G.²  Hall, A.C.¹  Thorne, S.D.² 

[1]University Laboratory of Physiology, Parks Road, Oxford, OX1 3PT, UK

[2]Department of Biochemistry and Molecular Biology, University of Manchester, Stopford Building, Oxford Road, Manchester, M13 9PT, UK

关键词: Glucose transport;    Hydrostatic pressure;    Red cell;    Facilitated diffusion;    Activation volume;    Membrane transport;    ZT;    zero-trans;    EE;    equilibrium exchanged;    ΔV*;    apparent activation volume;    FDNB;    1-fluoro-2;    4-dinitrobenzene;   

DOI  :  10.1016/0014-5793(92)80261-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the V _max but not significantly changing the K _m. The B _max and K _d of specific [³H]cytochalasin B binding were unaffected by pressure indicating no change to the number or affinity of functional transporters at pressure. Passive glucose transport was inhibited by pressure in a manner consistent with permeation across the lipid bilayer. These data indicate that there is a major change in volume during the translocation step of the glucose transporter which is rate-limiting for transport.

【 授权许可】

Unknown