Morphological alteration of Xenopus oocytes induced by valine‐14 p21rho depend on isoprenylation and are inhibited by Clostridium botulinum C3 ADP‐ribosyltranferase

FEBS Letters

Hall, Alan¹  Just, Ingo²  Mohr, Chistiane³  Aktories, Klaus³ 

[1] Institute of Cancer Research, Chester Beatty Laboratories, London, UK;Pharmakologisches Institut des Universitätsklinikums Essen, D-4300 Essen, FRG;Rudolph-Buchheim-Institut für Pharmakologie der Universität Gießen, D-6300 Gießen, FRG

关键词: Clostridium botulinum C3 ADP-ribosyltransferase;    Xenopus oocyte;    GTP-binding protein;    Rho;    Cytoskeleton;    Lovastatin;   

DOI  :  10.1016/0014-5793(90)81464-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microinjection of the constitutively active recombinant Val-14 p21rho A into Xenopus oocytes induced dramatic morphological changes with re-distribution of pigments from the animal pole resulting in spotted oocytes. The effects induced by Val-14 p21^{rho A} were regulated by progesterone in a dose-dependent manner whereas prior ADP-ribosylation of the rho protein blocked its activity. About 30 min after microinjection, p21 rho was associated with the plasma membrane. The membrane association of p21^rho and its biological activity were inhibited by lovastatin, an inhibitor of the 3-hydroxy-3-methylglutaryl coenzyme A reductase. The findings suggest that membrane attachment and biological activity of p21^rho depend on isoprenylation of the GTP-binding protein.

【 授权许可】

Unknown