Chloroquine inhibition of cholera toxin

FEBS Letters

Peterson, Johnny W.¹  Jackson, Christopher A.¹  Reitmeyer, James C.¹  Liang, Yi-fan¹ 

[1]Department of Microbiology, University of Texas Medical Branch, Galveston, TX 77550, USA

关键词: CT;    cholera toxin;    CQ;    chloroquine;    AA;    arachidonic acid;    cAMP;    3′;    5′-cyclic adenosine monophosphate;    PGs;    prostaglandins;   

DOI  :  10.1016/0014-5793(90)81459-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cholera toxin (CT) stimulated adenylate cyclase and a phospholipase which elevated levels of 3.5-cyclic adenosine monophosphate (cAMP) and arachidonic acid (AA). The AA was quickly converted to prostaglandins (PGs) via the cyclo-oxygenase pathway. Chloroquine exerted minimal inhibition of cAMP levels in CT-treated cells, although CT-induced release of [³H]AA and PGs was blocked completely when the drug was added in concentrations as low as 0.1 mM (30 μγ ml). Inhibition of [³H]AA release was complete when chloroquine was added before or within 30 min after CT. The capacity of chloroquine to inhibit either phospholipase C (PLC) or phospholipase A₂ (PLA₂) could explain the antisecretory activity of this drug.

【 授权许可】

Unknown