Phytochrome in lower plants Detection and partial sequence of a phytochrome gene in the moss Ceratodon purpureus using the polymerase chain reaction

FEBS Letters

Rüdiger, Wolfhart¹  Thümmler, Fritz¹  Beetz, Axel¹ 

[1] Botanisches Institut der Universität München, Menzingerstr. 67, 8000 München 19, FRG

关键词: Polymerase chain reaction;    Phytochrome;    Moss;    DNA sequence;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(90)81455-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The polymerase chain reaction was carried out with primers hybridizing to conserved regions of the phytochrome genes. With DNA from the moss Ceratodon purpureus 5 overlapping fragments were obtained resulting in a continuous nucleotide sequence of 1474 bp. The deduced amino acid sequence showed homology of around 60% with all known phytochrome sequences. The sequences contained a conserved chromophore attachment site. In light-grown Ceratodon protonemata the phytochrome mRNA with the size of about 4.5 kb was detected.

【 授权许可】

Unknown