Bidirectional promoter activity of the 5′ flanking region of the mouse thymidine kinase gene

FEBS Letters

Ogris, Egon¹  Wintersberger, Erhard¹  Weichslbraun, Irene¹ 

[1] Institut für Molekularbiologie der Universität Wien, Wasagasse 9, A-1090 Wien, Austria

关键词: Thymidine kinase;    Mouse libroblast;    Promoter activity;    CAT assay;    TK;    thymidine kinase;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/0014-5793(90)81436-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 5′ flanking region of the gene coding for cytoplasmic thymidine kinase (TK) in the mouse (a total of 490 bp upstream of the initiation codon) was tested for promoter activity using the chloramphenicol acetyltransferase gene as reporter. It was found that the region can be devided into two parts, one of which carries promoter activity in the direction of TK, whereas the 5′-half has promoter activity in the opossite direction. A fragment of 140 bp was sufficient for growth dependent promoter activity in the direction of TK, although about 100 bp further upstream, enhanced the activity. Expressions from the divergent promoter was independent of cell growth.

【 授权许可】

Unknown