Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection

FEBS Letters

Nakanishi, Mamoru¹  Noji, Masahide¹  Furuno, Tadahide¹  Ishihara, Minoru¹  Kawaura, Chiyo¹  Takeuchi, Ken-ichiro¹ 

[1] Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, Japan

关键词: Cationic liposome;    Gene transfection;    pSV2CAT DNA;    CAT assay;    Cationic cholesterol;    Zeta potential;   

DOI  :  10.1016/S0014-5793(96)01136-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cationic liposomes are known to be useful tools for gene transfection. However, the relation between transfection efficiency and physicochemical properties of liposomes has not been well understood. Here, we synthesized eight cationic derivatives of cholesterol which contain a tertiary amino head group with a different spacer arm. Transfection of plasmid pSV2CAT DNA into cells was done by cationic liposomes made of a mixture of dioleoylphosphatidylethanolamine (DOPE) and each cationic cholesterol derivative. At the same time we measured zeta potential of cationic liposomes by laser Doppler spectroscopy. The present results indicated that zeta potentials of cationic liposomes were well related to transfection activity of pSV2CAT DNA. This suggested that zeta potential of cationic liposomes is one of important factors which control gene transfection.

【 授权许可】

Unknown