Immunologically cross‐reactive and redox‐competent cytochrome b 6/f‐complexes in the chlorophyll‐free plasma membrane of cyanobacteria

FEBS Letters

Peschek, Günter A.¹  Wastyn, Marnik¹  Hager, Silvia¹  Kraushaar, Harald¹ 

[1] Biophysical Chemistry Group, Institute of Physical Chemistry, University of Vienna, Währingerstraβe 42, A-1090 Vienna, Austria

关键词: Respiratory chain;    Cytochrome b 6/f-complex;    Cytochrome-c reductase;    Inhibitor;    Plasma membrane;    Thylakoid membrane;    Cyanobacteria;    CM;    cytoplasmic (plasma) membrane;    ICM;    in-tracytoplasmic (thylakoid) membrane;    PCC;    Pasteur Culture Collection;    Paris;    France;    ATCC;    American Type Culture Collection;    cyt;    cytochrome;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(90)81091-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasma and thylakoid membranes were separated and purified from cell-free extracts of the cyanobacteria Anacystis nidulans, Synechocystis 6714, Anabaena variabilis and Nostoc sp. strain Mac. Immunoblots of the membrane proteins using antisera raised against subunits I–IV of the chloroplast b ₆/f-complex gave evidence for the presence of a homologous complex in both plasma and thylakoid membranes from the four species of cyanobacteria investigated. Both plasma and thylakoid membranes catalyzed the electron transfer from (exogenous) plastoquinol-9 and NADH to horse heart ferricytochrome c. However, while with plasma membranes these reactions were severely inhibited by low concentrations of antimycin A and rotenone, respectively, the inhibitors were without major effect on thylakoid membranes. The results will be discussed in terms of a possible similarity (analogy and/or homology?) of cyanobacterial plasma membranes to the inner mitochondrial membrane.

【 授权许可】

Unknown