Oxidation of methylamine by a Paracoccus denitrificans mutant impaired in the synthesis of the bc 1 complex and the aa 3‐type oxidase Evidence for the existence of an alternative cytochrome c oxidase in this bacterium

FEBS Letters

Oltmann, L.Fred¹  De Gier, Jan-Willem L.¹  Van Spanning, Rob J.M.¹  Stouthamer, Adriaan H.¹ 

[1] Biological Laboratory, Vrije Universiteit, Department of Microbiology, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

关键词: Methylamine oxidation;    Alternative cytochrome c oxidase;    Respiratory chain;    Paracoccus denitrificans;   

DOI  :  10.1016/0014-5793(92)80829-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Paracoccus denitrificans fbcC—ctaDII double mutant strain impaired in the synthesis of both the bc ₁ complex and the aa ₃-type oxidase has been constructed. This mutant strain, which is still able to grown on methylamine as sole carbon and energy source, exhibits unimpaired oxygen consumption with succinate, methylamine and endogenous substrates as electron donors. From kinetic studies of the oxidation and reduction rates of cytochromes c, it can be concluded that P. denitrificans contains a second cytochrome c oxidase, different from the aa ₃-type.

【 授权许可】

Unknown