Glucose‐stimulated efflux of indo‐1 from pancreatic β‐cells is reduced by probenecid

FEBS Letters

Berggren, Per-Olof¹  Nilsson, Thomas¹  Arkhammar, Per¹ 

[1] Department of Endocrinology, Karolinska Institute, Karolinska Hospital, Box 60 500, S-104 01 Stockholm, Sweden

关键词: Pancreatic β-cell;    Cytoplasmic free Ca2+;    Indo-1;    Probenecid;   

DOI  :  10.1016/0014-5793(90)81079-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Indo-1 loaded pancreatic β-cells, isolated from obese hyperglycaemic mice, were studied with respect to cytoplasmic free Ca²⁺ concentration ([Ca²⁺]_i), efflux of indicator and insulin release. In the absence of glucose there was a continuous efflux of indo-1 which increased upon stimulation with 20 mM of the sugar. The anion exchange inhibitor probenecid reduced both basal efflux of indo-1 and prevented that promoted by glucose. Measurements of [Ca²⁺]_i and insulin release revealed similar results as previously reported with quin-2 and fura-2. Furthermore, probenecid did not influence the [Ca²⁺]_i responses. It is thus possible to reduce efflux of indo-1 probenecid and thereby improve the measurements of [Ca²⁺]_iin pancreatic β-cells.

【 授权许可】

Unknown