期刊论文详细信息
Diseases of Aquatic Organisms
Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters
Seila Díaz1  Tristan Renault1  Antonio Villalba1  María Jesús Carballal1 
关键词: Disseminated neoplasia;    Cerastoderma edule;    Non-specific esterases;    Lysosome;    Respiratory burst;    Phagocytosis;    Cytoplasmic free Ca2+;    Actin;   
DOI  :  10.3354/dao02384
学科分类:生物科学(综合)
来源: Inter-Research
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【 摘 要 】

ABSTRACT: Disseminated neoplasia (DN) has been detected in cockles from various beds in Galicia (NW Spain). A study was performed to characterise cockle neoplastic cell ultrastructure and to evaluate the effect of this disease at different severity stages on various haemolymph cell parameters. Examination of cockle neoplastic cells with transmission electron microscopy (TEM) showed round shapes and a lack of pseudopods, a high nucleus:cytoplasm diameter ratio, Golgi complexes, abundant mitochondria, ribosomes, and numerous endoplasmic reticulum tubes and electron-lucent vesicles. Various haemolymph cell parameters (cell mortality, non-specific esterase and lysosome biovolume, reactive oxygen intermediates [ROI] production, phagocytosis ability, intracellular Ca2+ and actin levels) were compared between DN severity categories by flow cytometry; haemocyte mortality, non-specific esterase activities and lysosome biovolume were found to be higher with increasing DN severity. The phagocytic ability of neoplastic cells was sharply reduced with regard to haemocytes. The cytoplasmic-free Ca2+ level was higher and actin content lower in haemolymph cells of diseased cockles compared to unaffected ones. A significant increase in ROI production was detected in later stages of disease progression.

【 授权许可】

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