FEBS Letters | |
Synergy between zinc and phorbol ester in translocation of protein kinase C to cytoskeleton | |
Cowled, P.A.2  Zalewski, P.D.2  Forbes, I.J.2  Betts, W.H.1  Giannakis, C.2  | |
[1] Rheumatology Unit, The Queen Elizabeth Hospital, Woodville, South Australia 5011, Australia;Department of Medicine, University of Adelaide, Woodville, South Australia 5011, Australia | |
关键词: Zinc ionophore; Phorbol ester; Protein kinase C; Cytoskeleton; Zn; zinc; Ca; calcium; NP40; Nonidet P-40; PDBu; phorbol dibutyrate; PKC; protein kinase C; PMSF; phenylmethylsulphonyl fluoride; Phe; 1; 10-phenanthroline; | |
DOI : 10.1016/0014-5793(90)81067-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Protein kinase C was measured in the cytoskeletal fraction of lymphocytes, platelets and HL60 cells, by specific binding of [3H]phorbol dibutyrate and by immunoblotting with antibody to a consensus sequence in the regulatory domain of α-, β- and γ-isozymes of protein kinase C. Treatment of cells for 40 min with a combination of zinc (2–50 μM), zinc ionophore pyrithione and unlabelled phorbol dibutyrate (200 nM) caused up to a ten-fold increase in cytoskeletal protein kinase C and a corresponding decrease in other cellular compartments. Omission of any of the reagents resulted in much less or no translocation. These effects were inhibited by 1,10-phenanthroline, which chelates zinc, and were not seen with calcium. Increase in cytoskeletal protein kinase C persisted for several hours and appeared to involve attachment of the enzyme to actin microfilaments. We propose that zinc, like calcium, regulates the distribution of PKC in cells. However, unlike calcium which controls the binding of PKC to the lipid component on cell membranes, zinc controls the distribution of PKC to membrane cytoskeleton, possibly actin.
【 授权许可】
Unknown
【 预 览 】
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RO201912020294072ZK.pdf | 522KB | download |