Changes of fluorescence anisotropy in plasma membrane of human polymorphonuclear leukocytes during the respiratory burst phenomenon

FEBS Letters

Giorgi, Pier Luigi²  Curatola, Giovanna¹  Fiorini, Rosamaria¹  Bertoli, Enrico¹  Kantar, Ahmad² 

[1]Institute of Biochemistry, University of Ancona, Ancona 60100, Italy

[2]Pediatric Clinic, University of Ancona, Ancona 60100, Italy

关键词: Fluorescence anisotropy;    TMA-DPH;    Respiratory burst;    PMN;    PMNs;    polymorphonuclear leukocytes;    TMA-DPH;    1-(4-trimethylammoniumphenyl)-6-phenyl-1;    3;    5-hexatriene;    PMA;    phorbol myristate acetate;    FMLP;    N-formyl-methionyl-leucyl-phenylalanine;    PAF;    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine;    DPI;    diphenyl iodonium;   

DOI  :  10.1016/0014-5793(90)81065-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Steady state fluorescence anisotropy (r _s) of TMA-DPH was measured to study the effect of respiratory burst activation with PMA, FMLP, and PAF on the physico-chemical structure of PMNs plasma membrane. Our results show a significant increase in r _s during the respiratory burst activation. In the presence of NADPH-oxidase inhibitor DPI, only PAF induces changes in r _s values. This suggests a non-specific effect of PAF on plasma membrane. Azide, which induces a supranormal release of H₂O₂, fails to increase the basal r _s value after activation. Moreover, the catalase does not abolish the increase in rr _s induced upon activation. This rules out the possibility that changes of r _s during the respiratory burst activation are attributed mainly to H₂O₂ release. We conclude that multiple processes accompanying the respiratory burst activation are responsible for the changes in the physico-chemical properties of PMNs plasma membrane.

【 授权许可】

Unknown