Increased membrane heterogeneity in stimulated human granulocytes

FEBS Letters

Gratton, Enrico³  Governa, Mario²  Curatola, Giovanna¹  Fiorini, Rosamaria¹  Bertoli, Enrico¹  Valentino, Matteo² 

[1]Department of Physics, University of Illinois, Urbana, IL, USA

[2]Occupational Medicine Institute, Faculty of Medicine, University of Ancona, Ancona, Italy

[3]Biochemistry Institute, Faculty of Medicine, University of Ancona, Ancona, Italy

关键词: Membrane heterogeneity;    Microdomain;    Fluorescence lifetime;    1-(4-Trimethylammoniumphenyl)-6-phenyl-1;    3;    5-hexatriene;    (Granulocyte);    PMN;    polymorphonuclear leukocyte;    TMA-DPH;    1-(4-trimethylammoniumphenyl)-6-phenyl-1;    3;    5-hexatriene;    FMLP;    N-formyl-methionyl-leucyl-phenylalanine;    HBSS;    Hanks' balanced salt solution without red phenol;   

DOI  :  10.1016/0014-5793(88)80136-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

TMA-DPH fluorescence decay in human PMN before and after stimulation with FMLP was studied using frequency domain fluorometry. Membrane heterogeneity was assessed by the width of the continuous distributions of lifetime values of Lorentzian shape used to describe the fluorescence decay. In non-stimulated granulocytes TMA-DPH fluorescence decay is characterized by two distributions of lifetime values centered at 6.5 and 1.0 ns and full width at half maximum of 0.3 and 1.2 ns, respectively. Within 15 min after stimulation, the center values of the two distribution components were 5.1 and 0.8 ns and the distribution width was 0.8 and 0.6 ns, respectively. These results indicate changes of membrane domain organization which can be ascribed to compositional changes redistribution of membrane components.

【 授权许可】

Unknown