The reaction specificities of the pea and a cyanobacterial thylakoid processing peptidase are similar but not identical

FEBS Letters

Wallace, T.P.²  Howe, C.J.²  Robinson, C.¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK;Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK

关键词: Thylakoid lumenal protein;    Leader sequence;    Processing peptidase;    Cyanobacteria;    Pea;   

DOI  :  10.1016/0014-5793(90)80468-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thylakoid processing peptidase from the cyanobacterium Phormidium laminosum has been extracted from thylakoid membranes by solubilization with Triton X-100. Its reaction specificity has been compared with the analogous pea peptidase by processing in vitro of radiolabelled wheat and P. laminosum thylakoid lumenal precursor polypeptides. The cyanobacterial polypeptide is processed to the mature size through an intermediate by the P. laminosum peptidase, but to a polypeptide that has a slightly greater apparent molecular weight than the intermediate by the pea peptidase. Both peptidases correctly process the wheat polypeptide. This suggests that the reaction specificities of the two peptidases are similar, but not identical.

【 授权许可】

Unknown