Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence

FEBS Letters

Maeda, Kayo¹  Oki, Manami¹  Maéda, Yuichiro¹  Sano, Ken-Ichi¹ 

[1] RIKEN Harima Institute at SPring-8, Laboratory for Structural Biochemistry, Mikazuki, Sayo, Hyogo 679-5148, Japan

关键词: Baculovirus;    Sf9;    Recombinant protein expression;    Leader sequence;    Tropomyosin;    L21;    21 bp 5′ untranslated leader sequence of a lobster tropomyosin cDNA;    UTR;    untranslated region;    AcNPV;    Autographa californica nuclear polyhedrosis virus;    RαTm;    rabbit skeletal muscle α-tropomyosin;    sTm1;    lobster slow muscle-specific tropomyosin type 1 isoform;   

DOI  :  10.1016/S0014-5793(02)03659-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe a cis element that dramatically increases the expression levels of exogenous genes in baculovirus-infected insect cells. This 21 bp sequence element is derived from a 5′ untranslated leader sequence of a lobster tropomyosin cDNA (L21). By using a transfer vector carrying L21, the expression levels of tropomyosin and luciferase were 20- and seven-fold higher with L21 than without L21, respectively. L21 has both the Kozak sequence and the A-rich sequence found in the polyhedrin leader sequence. We assume that both sequence elements are essential for the enhancement of protein expression in the baculovirus-based expression system.

【 授权许可】

Unknown