EGF stimulates the processing and export of a secreted form of EGF receptor

FEBS Letters

Das, Manjusri¹  Khire, Jayant¹ 

[1] Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA

关键词: EGF receptor;    Glycoprotein processing;    Protein secretion;    Biosynthetic transport;    EGF;    epidermal growth factor;    DME-medium;    Dulbecco's modified Eagle's medium;    FBS;    fetal bovine serum;    ER;    endoplasmic reticulum;   

DOI  :  10.1016/0014-5793(90)80451-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human A431 cells express a 100 kDa EGF-receptor homolog that contains only the external domain. The kinetics of its maturation and export are slow and comparable to those of the transmembrane receptor. Here we demonstrate that exogenously added EGF stimulates post-translational processing and export of this receptor through a pathway that is insensitive to inhibitors of protein synthesis. The results suggest that EGF may influence one or more of the rate determining steps that control receptor export from endoplasmic reticulum. This may constitute one of the path-ways by which EGP regulates the expression of its receptor.

【 授权许可】

Unknown