1‐Deoxymannojirimycin inhibits Golgi‐mediated processing of glycoprotein in Xenopus oocytes

FEBS Letters

Zoppè, Monica¹  Fabbrini, Maria Serena¹  Vitale, Alessandro¹  Bollini, Roberto¹ 

[1] Istituto Biosintesi Vegetali, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy

关键词: 1-Deoxymannojirimycin;    Oocyte;    Glycoprotein processing;    Phytohemagglutinin;    Golgi complex;    (Xenopus laevis);   

DOI  :  10.1016/0014-5793(88)80144-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We prepared in vitro an mRNA transcript coding for the erythroagglutinating subunit of the kidney bean glycoprotein phytohemagglutinin, E-PHA. The mRNA, injected into Xenopus oocytes, synthesized E-PHA carrying two Asn-linked carbohydrate chains, one of which was processed and acquired resistance to endo-β-N-acetylglucosaminidase H, as occurs in the native bean cells. When the mannose analog 1-deoxymannojirimycin, an inhibitor of mammalian Golgi mannosidase I, was included in the oocyte culture medium, the acquisition of endo-β-N-acetylglucosaminidase H resistance was abolished, indicating that also in an amphibian cell the inhibitor blocks a key reaction in Golgi-mediated processing.

【 授权许可】

Unknown