Importance of the G27‐A43 mismatch at the anticodon stem of Escherichia coli tRNAThr2

FEBS Letters

Inokuchi, Hachiro¹  Komine, Yuriko¹ 

[1] Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan

关键词: E. coli;    tRNAThr;    Amber suppressor;    G27-A43 mismatch;   

DOI  :  10.1016/0014-5793(90)80447-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tRNA^Thr₂ isoacceptor of E. coli has a G-A mismatch at positions 27–43. When the anticodon of this tRNA was converted to an amber anticodon (CUA), this tRNA showed suppressor activity in E. coli. Moreover, introduction of the base pair (G-C or U-A) at positions 27–43 of this suppressor tRNA reduced its suppressor activity. These results indicate that the G27-A43 mismatch is necessary for full function of tRNA^Thr₂.

【 授权许可】

Unknown