Functional expression of the mutants of the chloroplast tRNALys gene from the liverwort, Marchantia polymorpha, in Escherichia coli

FEBS Letters

Inokuchi, Hachiro¹  Ozeki, Haruo¹  Nakahigashi, Kenji¹ 

[1] Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606, Japan

关键词: Chloroplast tRNA gene;    Marchantia polymorpha;    Amber suppressor;    C27-C43 mismatch;    ssDNA;    single-stranded DNA;    IBS;    intron binding sequence;    EBS;    exon binding sequence;   

DOI  :  10.1016/0014-5793(90)80883-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The anticodon of the tRNA^Lys gene (trnK) in the liverwort, Marchantia polymorpha, was anificially converted to an amber anticodon. This mutant tRNA^Lys(CTA) gene carrying either the intron or the C₂₇-C₄₃ mismatch at the anticodon-stem is not functional in Escherichia coli, but without both of them, it does work as a tRNA^Lys amber suppressor.

【 授权许可】

Unknown