Evidence for communication between nerve growth factor and protein tyrosine phosphorylation

FEBS Letters

Harmar, Tony²  Tonks, Nicholas K.¹  Gómez, Néstor³  Morrison, Christine²  Cohen, Philip³ 

[1]Department of Biochemistry, University of Washington, Seattle, WA, USA

[2]MRC Brain Metabolism Unit, University of Edinburgh, Edinburgh, UK

[3]Department of Biochemistry, University of Dundee, Dundee, UK

关键词: Nerve growth factor;    Tyrosine phosphorylation;    Protein phosphatase;    Okadaic acid;    Protein kinase;    PC12 cell;   

DOI  :  10.1016/0014-5793(90)80386-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nerve growth factor (NGF) stimulation of PC12 cells activated two myelin basic protein (MBP) kinase activities > 10-fold within 5 min, which were resolved by chromatography on Mono Q. Each enzyme phosphorylated MBP on threonine and was inactivated by incubation with either CD45, a protein tyrosine phosphatase, or protein phosphatase 2A (PP2A), a serine/threonine phosphatase. The effects of CD45 and PP2A were prevented by vanadate and okadaic acid, respectively. Activation of the MBP-kinases provides a mechanism for communication between NGF and intracellular protein tyrosine phosphorylation.

【 授权许可】

Unknown