FEBS Letters | |
Specific binding of sulphated polymers to ram sperm proacrosin | |
Jones, Roy1  Williams, Rachel M.1  | |
[1] AFRC Institute of Animal Physiology and Genetics Research, Babraham Hall. Cambridge CB2 4 AT, UK | |
关键词: Proacrosin; Sulphate group; Adhesion molecule; Zona pellucida; Fertilization; GLC; gas liquid chromatography; BSA; bovine serum albumin; SDA-PAGE; sodium dodecyl sulphate polyacrylamide gel electrophoresis; PBS; phosphate buffered saline pH 7.2; CNBr; cyanogen bromide; | |
DOI : 10.1016/0014-5793(90)81259-Q | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Sulphated polysaccharides and zona pellucida glycoproteins have been shown to bind non-enzymatically to proacrosin, the protein found within the acrosomal vesicle of mammalian spermatozoa. The mechanism of this interaction has been investigated using 125I-fucoidan to probe purified ram sperm proacrosin. Results show that (a) binding of' 125I-fucoidan to proacrosin is inhibited only by sulphated polymers and (b) recognition is mediated by poly(sulphate) groups and is largely independent of the composition of the polymer chain. It is suggested that a similar mechanism is responsible for the interaction between proacrosin and zona pellueida glycoproteins during the early stages of fertilization in mammals and this process mediates firm binding of spermatozoa to the egg.
【 授权许可】
Unknown
【 预 览 】
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