Effect of canine surfactant protein (SP‐A) on the respiratory burst of phagocytic cells

FEBS Letters

Heilmann, Peter¹  Meyer, Barbara¹  Maier, Konrad L.¹  Weber, Hans¹ 

[1] Gesellschaft für Strahlen- und Umweltforschung, Projekt Inhalation, Ingolstädter Landstrasse 1, D-8042 Neuherberg, FRG

关键词: Surfactant;    Surfactant associated protein A;    Respiratory burst;    Chemiluminescence;    Neutrophil;    Alveolar macrophage;    Beagle dog;    SP-A;    surfactant associated protein A (28;    000–36;    000 Da);    CL;    Chemiluminescence;    PMA;    phorbol-12-myristate-13-acetate;    BAL;    bronchoalveolar lavage;   

DOI  :  10.1016/0014-5793(90)81241-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cells obtained from bronchoalveolar lavage, or neutrophils of peripheral blood of dog, were incubated with the canine surfactant-associated protein A (SP-A). A significant decrease of the production of Superoxide anion was observed after subsequent stimulation with phorbol-12-myristate-13-acetate (PMA) as measured by the lucigenin-dependent chemiluminesence (CL). Several other proteins used for control experiments did not decrease lucigenin-dependent CL, indicating a specific effect of SP-A on phagocytes. Treatment of SP-A with collagenase prior to incubation with neutrophils destroyed the depleting effect on oxygen radical production of PMA-stimulated cells. We propose that SP-A acts as a regulatory factor of the respitatory burst of alveolar macrophages and neutrophils in the lungs. The inhibitory effect of SP-A is down-regulated by collagenase released from stimulated alveolar macrophages.

【 授权许可】

Unknown