Inhibition of thrombin by synthetic hirudin peptides

FEBS Letters

Hermans, Jan²  Binnie, Cameron G.²  Erickson, Bruce W.¹ 

[1] Department of Chemistry, A808 Kenan Labs., University of North Carolina, Chapel Hill, NC 27599, USA;Department of Biochemistry, 323 Faculty Laboratory Office Building Chapel Hill, NC 27599, USA

关键词: Hirudin;    Thrombin;    Synthetic segment;    Peptide segment;   

DOI  :  10.1016/0014-5793(90)81240-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the role of different regions of hirudin in the interaction with the proteinase thrombin, segments of hirudin containing 15-51 residues were synthesized. The C-terminal segment 40-65 inhibited the fibrinogen clotting activity of thrombin but not amidolysis of tosyl-Gly-Pro-Arg-p-nitroanilide. Central peptide 15–42 was insoluble at pH 7, but peptide 15-65 inhibited fibrinogen clotting and amidolysis to an equal extent. The N-terminal loop peptide 1-15 had no inhibitory activity and did not affect the potency of peptide 15-65. These data suggest that the central region inhibits catalysis.

【 授权许可】

Unknown