Expression of the cholecystokinin gene in a human (small‐cell) lung carcinoma cell‐line

FEBS Letters

Folkesson, Ronnie¹  Geijer, Thomas²  Rehfeld, Jens F.¹  Monstein, Hans-Jürg¹ 

[1]Department of Clinical Biochemistry (KK6321), State University Hospital (Rigshospitalet), DK-2JOO Copenhagen, Denmark

[2]Department of Pharmacology, Biomedicum, S-751 24 Uppsala, Sweden

关键词: Cholecystokinin;    Gastrin;    Opioid peptide;    Gene expression;    Neuronal cell-line;    Northern blot;   

DOI  :  10.1016/0014-5793(90)81227-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of the cholecystokinin (CCK), gastrin and enkephalin A genes were studied by Northern blot analysis and a library of sequence-specific radioimmunoassays in human cell lines. The human small-cell lung carcinoma line (SCLC) U-1690 expressed moderate levels of CCK mRNA as compared to the human neuroepithelioma cell line SK-N-MC. Neither gastrin nor (pro)enkephalin A mRNAs were detectable in the U-1690 cell line. In contrast, the SCLC-line H-69 expressed Enk A but no CCK mRNA. The radioimmunoassays showed that the CCK mRNA transcript in the SCLC line U-1690 also is translated, and that preproCCK is processed into bioactive, carboxyamidated CCK peptides. Thus, the human small cell carcinoma cell line U-1690 is a useful model for studies of cell-specific CCK gene expression.

【 授权许可】

Unknown