Transient shift of diacylglycerol and inositol lipids induced by interferon in Daudi cells

FEBS Letters

Cataldi, A.¹  Miscia, S.¹  Lisio, R.²  Cocco, L.¹  Rana, R.¹ 

[1] Istituto di Morfologia Umana Normale, Università di Chieti Chieti, Italy;Istituto di Citomorfologia Normale e Patologica del CNR, Chieti, Italy

关键词: Interferon;    Diacylglycerol;    Inositol lipid;    Daudi cell;    DAG;    diacylglycerol;    PIP2;    phosphatidylin.ositol 4;    5-bisphosphate;    IP3;    inositol 1;    4;    5 trisphosphate;    rIFNA;    recombinant DNA human interferon A;    PMSF;    phenylmethanesulfonylfluoride;    TLC;    thin layer chromatography;    LPL;    less polar lipids;   

DOI  :  10.1016/0014-5793(90)81216-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of human recombinant DNA interferon-α type A on inositol lipid and diacylglycerol metabolism was investigated in Daudi lymphoma whole cells and isolated nuclei. In isolated nuclei after 90 min of interferon treatment an enhanced rate of PIP₂ phosphorylation and an increase of DAG mass were observed. In whole cells, after 1 min of interferon treatment, there was a rapid and transient shift of DAG mass apparently not related to inositol lipid modifications, thus indicating the presence in nuclear and cytoplasmic compartments of inositol lipid fractions with different metabolic features in response to interferon-α.

【 授权许可】

Unknown