DNA methylation inhibits transcription by RNA polymerase III of a tRNA gene, but not of a 5S rRNA gene

FEBS Letters

Wagner, Herbert¹  Simon, Dietrich¹  Besser, Daniel¹  Götz, Frank¹  Kröger, Hans¹  Schulze-Forster, Kai¹ 

[1] Robert Koch-Institut, Abteilung Biochemie, Nordufer 20, D-1000 Berlin 65, Germany

关键词: DNA methylation;    RNA polymerase III;    tRNAlysgene;    5S rRNA gene;    Transcription;    Oocyte;    TFIIIA/B/C;    transcription factors A/B/C for polymerase III;    SAM;    S-adenosyl-L-methionin;    NP;    nucleotide position;   

DOI  :  10.1016/0014-5793(90)81193-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methylation of cytosine in the DNA inhibits the transcription by RNA polymerase II in higher eukaryotes, but has no influence on RNA polymerase I transcription. The effect on RNA polymerase III was unknown, so far. Two polymerase III genes: a type 1 5S rRNA gene and a type 2 tRNA gene were methylated in vitro with a purified eukaryotic DNA methyltransferase (EC2.1.1.37) and their transcription was analyzed in Xenopus oocytes. The 5S rRNA gene, an oocyte 5S rRNA gene from X. laevis which is subject to developmental inactivation, was not affected by methylation. Conversely, transcription of the tRNA gene was 80% inhibited by methylation with the eukaryotic methyltransferase. HhaI and HpaII methylation left its transcription unaffected.

【 授权许可】

Unknown