The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors

Cellular & Molecular Biology Letters

Giorgio Dieci¹  Roberto Ferrari¹ 

[1] Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Parma, Italy$$

关键词: RNA polymerase III;    Transcription reinitiation;    TFIIIB;    G-less cassette;   

DOI  :  10.2478/s11658-007-0041-y

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Transcription reinitiation by RNA polymerase (Pol) III proceeds through facilitated recycling, a process by which the terminating Pol III, assisted by the transcription factors TFIIIB and TFIIIC, rapidly reloads onto the same transcription unit. To get further insight into the Pol III transcription mechanism, we analyzed the kinetics of transcription initiation and reinitiation of a simplified in vitro transcription system consisting only of Pol III and template DNA. The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate, while facilitated reinitiation during subsequent cycles is compromised.

【 授权许可】

Unknown