FEBS Letters | |
Cloning of cDNA and genomic DNA for human cytochrome P‐45011β | |
Hosoda, Kiminori3  Imura, Hiroo3  Kawamoto, Takeshi1  Miyahara, Kaoru1  Toda, Katsumi1  Mitsuuchi, Yasuhiro1  Yokoyama, Yuichi2  Nakao, Kazuwa3  Shizuta, Yutaka1  Yamamoto, Yasutake2  | |
[1] Department of Medical Chemistry, Kochi Medical School, Nankoku, Kochi 783 Japan;Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi 783 Japan;Second Division, Department of Medicine, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606, Japan | |
关键词: Monooxygenase; P-45011β; cDNA; Genomic DNA; Adrenal tumor; Steroidogenesis; | |
DOI : 10.1016/0014-5793(90)81190-Y | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A full-length cDNA clone encoding steroid 11β-hydroxylase (P-45011β) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-45011β gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-45011β gene.
【 授权许可】
Unknown
【 预 览 】
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