Cloning of cDNA and genomic DNA for human cytochrome P‐45011β

FEBS Letters

Hosoda, Kiminori³  Imura, Hiroo³  Kawamoto, Takeshi¹  Miyahara, Kaoru¹  Toda, Katsumi¹  Mitsuuchi, Yasuhiro¹  Yokoyama, Yuichi²  Nakao, Kazuwa³  Shizuta, Yutaka¹  Yamamoto, Yasutake² 

[1] Department of Medical Chemistry, Kochi Medical School, Nankoku, Kochi 783 Japan;Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi 783 Japan;Second Division, Department of Medicine, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606, Japan

关键词: Monooxygenase;    P-45011β;    cDNA;    Genomic DNA;    Adrenal tumor;    Steroidogenesis;   

DOI  :  10.1016/0014-5793(90)81190-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA clone encoding steroid 11β-hydroxylase (P-450_11β) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-450_11β gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-450_11β gene.

【 授权许可】

Unknown