Abnormal behavior of protein kinase C in the human myeloma cell line, RPMI 8226

FEBS Letters

Vial, Henri¹  Parant, Marc R.¹  Klein, Bernard² 

[1] CNRS URA 530, Interactions membranaires, Université Montpellier II, case 107, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France;NSERM U 291, Zolad, 34100 Montpellier Cedex, France

关键词: Myeloma cell line;    RPMI 8226;    B lymphocyte;    Protein kinase C;    DAG;    diacylglycerol;    E 64;    trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane;    PDBu;    phorbol 12;    13-dibutyrate;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;   

DOI  :  10.1016/0014-5793(90)81187-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase C activity of the human myeloma cell line, RPMI 8226, was studied after prepurification on DEAE-cellulose. The total protein kinase activity, eluted at 0.12 M NaCl, was 493 cells, but 38% was associated with membranes. The lipid dependence of cytosolic and membrane activities was only 52% and 21%, respectively. This activity increased with time, to as much as 200% for the membrane fraction after 7 days, whereas lipid dependence and the PDBu binding properties were lost. This modified activity was not due to the extinction of a copurifying endogenous inhibitor nor to classical PKC proteolysis. TPA-treatment of these eels is accompanied by a rapid, selective and complete loss of lipid-dependent activity of the cytosol, thus benefiting co-migrating lipid independent activity, with no membrane fraction recovery or PKM formation.

【 授权许可】

Unknown