Synergistic and additive induction of metallothionein in Chang liver cells

FEBS Letters

Kosaka, Futami¹  Denry Sato, J.²  Yamada, Teruo¹  Fukushima, Tomihiro¹  Bhuiyan, Lutful A.¹  Iijima, Yoshio¹ 

[1] Department of Anesthesiology and Resuscitology, Okayama University Medical School, 2-5-1 Shikata-Cho, Okayama, 700 Japan;W. Alton Jones Cell Science Center, 10 Old Barn Road, Lake Placid, NY 12946, USA

关键词: Synergistic induction;    Additive induction;    Metallothionein;    MT;    metallothionein;    EGF;    epidermal growth factor;    TGF-α;    transforming growth factor-α;    Dex;    dexamethasone;   

DOI  :  10.1016/0014-5793(90)81158-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied metallothionein (MT) induction by three primary inducers; a heavy metal, a glucocorticoid and a cytokine, and by the combinations of these inducers in the cultured cells. MT-protein was synergistically induced by either a cytokine or a heavy metal with a glucocorticoid hormone and was additively induced by the combination of a cytokine and a heavy metal, but MT-mRNA levels were not completely correlated with MT-protein levels. These results suggest that posttranscriptional regulation may be involved in the synergistic induction of MT-protein. We propose a possible mechanism in which marked MT induction by stress in vivo is dependent on the combined effect of two or more inducers, because marked MT induction is not seen by an injection of a plausible dose of either a glucocorticoid hormone or a cytokine in vivo.

【 授权许可】

Unknown