Production of enkephalin in tobacco protoplasts using tobacco mosaic virus RNA vector

FEBS Letters

Meshi, Tetsuo¹  Okada, Yoshimi¹  Takamatsu, Nobuhiko³  Yanagi, Hideki²  Shiba, Tadayoshi³  Watanabe, Yuichiro¹ 

[1] Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan;Biotechnology Laboratory, Takarazuka Research Center, Swnitomo Chemical Co., Ltd., 4-2-1 Takatsukasa, Takarazuka, Hyogo 665, Japan;Laboratory of Molecular Biology. School of Hygienic Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228, Japan

关键词: Enkephalin;    Fusion protein;    Transcription system;    in vitro;    RNA vector;    Tobacco mosaic virus;   

DOI  :  10.1016/0014-5793(90)81121-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To examine the validity of the strategy to express a foreign gene as a fusion protein with the coat protein (CP) of tobacco mosaic virus (TMV), we have constructed ENK RNA by using an in vitro transcription system of TMV RNA. ENK RNA differs from TMV RNA only in that ENK RNA carries an additional sequence coding for Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) (Enk) with a preceding in-frame methionine just before the termination codon of CP gene. In protoplasts inoculated with ENK RNA, CP+Enk fusion protein accumulated as the major protein.

【 授权许可】

Unknown