Human μ opiate receptor

FEBS Letters

Hawkins, Anita L.²  Wang, Jia-Bei¹  Uhl, George R.¹  Griffin, Constance A.²  Persico, Antonio M.¹  Johnson, Peter S.¹ 

[1] Molecular Neurobiology Branch, Addiction Research Center, NIDA, Baltimore, MD, USA;Oncology Center and Neuroscience, Johns Hopkins School of Medicine, USA

关键词: Morphine;    Heroin;    Pain;    Addiction;    Enkephalin;    G-linked receptor;   

DOI  :  10.1016/0014-5793(94)80368-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24–25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.

【 授权许可】

Unknown