FEBS Letters | |
Arachidonic acid epoxygenase | |
Dishman, Elizabeth2  Karara, Armando2  Falck, J.R.1  Jacobson, Harry2  Capdevila, Jorge H.2  | |
[1] Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75235, USA;Division of Nephrology, Vanderbilt University Medical School, Nashville, TN 37232, USA | |
关键词: Cytochrome P-450; Epoxygenase; Arachidonic acid; Epoxyeicosatrienoic acid; EETs; cis-epoxyeicosatrienoic acids; EET-Me; methyl-cir-epoxyeicosatrienoate; EET-PFB; pentafluorobenzyl-cis-epoxyeicosatrienoate; HPLC; high-pressure liquid chromatography; GC; gas-liquid chromatography; NICI/MS; electron capture negative ion chemical ionization mass spectrometry; | |
DOI : 10.1016/0014-5793(90)81014-F | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.
【 授权许可】
Unknown
【 预 览 】
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RO201912020293671ZK.pdf | 424KB | download |