Arachidonic acid epoxygenase

FEBS Letters

Dishman, Elizabeth²  Karara, Armando²  Falck, J.R.¹  Jacobson, Harry²  Capdevila, Jorge H.² 

[1] Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75235, USA;Division of Nephrology, Vanderbilt University Medical School, Nashville, TN 37232, USA

关键词: Cytochrome P-450;    Epoxygenase;    Arachidonic acid;    Epoxyeicosatrienoic acid;    EETs;    cis-epoxyeicosatrienoic acids;    EET-Me;    methyl-cir-epoxyeicosatrienoate;    EET-PFB;    pentafluorobenzyl-cis-epoxyeicosatrienoate;    HPLC;    high-pressure liquid chromatography;    GC;    gas-liquid chromatography;    NICI/MS;    electron capture negative ion chemical ionization mass spectrometry;   

DOI  :  10.1016/0014-5793(90)81014-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.

【 授权许可】

Unknown