Hydrogen peroxide production during experimental protein glycation

FEBS Letters

Wolff, Simon P.¹  Woollard, Alison C.S.¹  Jiang, Zhen-Yue¹ 

[1] Toxicology Laboratory, Department of Clinical Pharmacology, University College London, 5 University St., London WC1E 6JJ, UK

关键词: Hydrogen peroxide;    Xylenol orange;    Glucose autoxidation;    Protein glycation;   

DOI  :  10.1016/0014-5793(90)80974-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The accumulation of hydrogen peroxide (H₂O₂) during incubations of protein with glucose (experimental glycation) has previously been too low for direct measurement although it is suggested to be the precursor of protein-damaging hydroxylating agents. We have thus developed a simple H₂O₂-measuring technique which relies upon the rapid peroxide-mediated oxidation of Fe²⁺ to Fe³⁺ (catalysed by sorbitol) under acidic conditions followed by reaction of the latter cation with the dye, xylenol orange. We have used the method to demonstrate that incubation mixtures of protein and glucose generates nanomolar levels of hydrogen peroxide in the presence of protein under physiological conditions of pH and temperature.

【 授权许可】

Unknown