Expression of phosphoglycerate mutase mRNA in differentiating rat satellite cell cultures

FEBS Letters

Climent, F.²  Castellà-Escolà, J.²  Carreras, J.²  Ureña, J.²  Alterio, J.¹  Martelly, I.¹ 

[1]Laboratoire de Myogenése et Régéneration Musculaire (MY REM), UFR Sciences et Technologie, Université Paris-Val de Marne, Créteil, France

[2]Unitat de Bioquimica, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain

关键词: Phosphoglycerate mutase;    Cell culture;    Isozyme transition;    mRNA;   

DOI  :  10.1016/0014-5793(90)80963-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Poly (A)⁺ mRNA was isolated from rat satellite cell cultures and analyzed by Northern blot analyses for mRNA content of phosphoglycerate mutase (PGAM) isozymes. In non-differentiating satellite cells only PGAM-B mRNA was detected, but when cells were differentiated into myotubes, which undergo spontaneous contraction, mRNA for PGAM-M muscle-specific isozyme was also detected. This finding is in perfect concordance with the transition of PGAM isozymes encountered in the same cell cultures, and strongly supports a transcriptional control of PGAM expression throughout myogenesis independently of nerve influence.

【 授权许可】

Unknown