Differential expression of laminin chains in hepatic lipocytes

FEBS Letters

Guillouzo, André¹  Loreal, Olivier¹  Rescan, Pierre-Yves¹  Yamada, Yoshihiko²  Clement, Bruno¹  Levavasseur, Françoise¹ 

[1] Unité de Recherches Hépatologiques U 49 de l'INSERM, Hôpital de Pontchaillou, 35033 Rennes, France;Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda MD 20892, USA

关键词: Laminin;    Lipocyte;    Liver;    Cell culture;    mRNA;   

DOI  :  10.1016/0014-5793(91)81213-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lipocyte is an important source of laminin in the normal liver. We have investigated the expression of the 3 chains of laminin in isolated rat lipocytes. Both B₁ and B₂ chains, but not A, were found in medium from 5-day-old lipocyte primary cultures by immunoblotting and immunoprecipitation of ³⁵S-labeled proteins after reducing SDS-polyacrylamide gel electrophoresis. An additional polypeptide of M _r=380 000 was identified by immunoprecipitation. Under non-reducing conditions only one M _r=900 000 band was revealed. High levels of B₁ and B₂ mRNAs were also demonstrated in 5-day-old cultured lipocytes while at the time of seeding, only B₂ chain mRNAs were clearly detectable. A chain mRNA was constantly absent. These results suggest that lipocytes produce a variant form of laminin in primary culture and that the M _r=380 000 polypeptide could be unrelated to the A chain of laminin.

【 授权许可】

Unknown