Apis mellifera cytoplasmic elongation factor 1α (EF‐1α) is closely related to Drosophila melanogaster EF‐1α

FEBS Letters

Hovemann, Bernhard T.¹  Walldorf, Uwe² 

[1] Center for Molecular Biology Heidelberg (Z.M.B.H.), Im Neuenheimer Feld 282, D-6900 Heidelberg, FRG;Biocenter of the University of Basel, Klingelbergstraße 70, CH-4056 Basel, Switzerland

关键词: Protein synthesis;    Elongation factor;    Translation;    Genomic sequence;    Apis mellifera;    Drosophila melanogaster;   

DOI  :  10.1016/0014-5793(90)80936-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using low stringency hybridisation with a Drosophila melanogaster EF-1α gene fragment we have isolated a genomic DNA clone encoding elongation factor 1α (EF-1α) from Apis mellifera. The hybridising Apis mellifera sequence could be delineated to two small EcoRI fragments that were also revealed by genomic Southern hybridisation. By comparison with the corresponding Drosophila melanogaster data the complete translational reading frame has been deduced. It is interrupted by two intervening sequences of 220 and about 790 nucleotides. Comparison with known eucaryotic EF-1α sequences further confirms that certain amino acid sequences seem to be invariable within the EF-1α protein family.

【 授权许可】

Unknown