Electron microscopy of native and artificial methylreductase high‐molecular‐weight complexes in strain Gö 1 and Methanococcus voltae

FEBS Letters

Mayer, F.¹  Hoppert, M.¹ 

[1] Institut für Mikrobiologie, Grisebachstr. 8, D-3400 Göttingen, FRG

关键词: Methanogenic bacterium;    Inside-out vesicle;    Methyl-CoM methylreductase (component C);    Methanoreductosome;    Partial reconstitution;    Electron microscopy;   

DOI  :  10.1016/0014-5793(90)80281-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The preparation of inside-out vesicles from methanogenic bacteria with protein cell walls was improved with regard to the preservation of structure and localization of membrane-bound proteins. Complexes similar to the methanoreductosome in the methanogenic bacterium Gö 1 [1] were also found attached to the inner aspect of the cytoplasmic membrane of Methanococcus voltae. Methanoreductosomes were purified from crude extracts of Gö 1-cells by affinity chromatography. Under specific conditions at high protein concentrations methyl-CoM-methylreductase molecules isolated from Gö 1-cells could be reassociated to spherical complexes of various sizes, with an appearance similar to that of methanoreductosomes isolated from strain Gö 1.

【 授权许可】

Unknown