ATP synthesis coupled to electron transfer from H2 to the heterodisulfide of 2‐mercaptoethanesulfonate and 7‐mercaptoheptanoylthreonine phosphate in vesicle preparations of the methanogenic bacterium strain Gö1

FEBS Letters

Thauer, R.K.¹  Hedderich, R.¹  Blaut, M.²  Peinemann, S.²  Gottschalk, G.² 

[1]Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität Marburg, D-3550 Marburg, FRG

[2]Institut für Mikrobiologie der Georg-August-Universität Göttingen, D-3400 Göttingen FRG

关键词: ATP synthesis;    Heterodisulfide reductase;    Methanogenic bacterium;    Vesicle preparation;    BES;    2-bromoethanesulfonic acid;    CoM-SH;    2-mercaptoethanesulfonate;    CoM-S-S-HTP;    heterodisulfide of CoM-SH and HTP-SH;    DCCD;    N;    N'-dicyclohexylcarbodiimide;    DES;    diethylstilbestrol;    HTP-SH;    7-mercaptoheptanoylthreonine phosphate;    CH3-S-CoM or methyl-CoM;    2-(methylthio)ethanesul-fonate;    SF 6847;    3;    5-di-tert-butyl-4-hydroxybenzylidenemalono-nitrile;    sulfobetaine;    N-tetradecyl-N;    N-dimethylammonio-3-propanesulfonate;   

DOI  :  10.1016/0014-5793(90)80704-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Crude vesicle preparations of the methanogenic strain Gol were able to couple the reduction of the heterodisulfide of 2-mercaptoethanesulfonate and 7-mercaptoheptanoylthreonine phosphate (CoM-S-S-HTP) by H₂ with ATP formation. The rate of ATP synthesis was 1 mg protein. ATP synthesis and disulfide reduction were only observed with CoM-S-S-HTP, but not with CoM-S-S-CoM or HTP-S-S-HTP. The methylreductase inhibitor 2-bromoethanesulfonic acid had no effect on ATP synthesis induced by CoM-S-S-HTP reduction with H₂. ATP synthesis was completely inhibited by the uncoupler SF 6847 whereas the concomitant CoM-S-S-HTP reduction was stimulated. The ATP synthase inhibitors DCCD and DES also inhibited ATP formation completely and decreased the CoM-S-S-HTP reduction rate to 35% of the control. These inhibitory effects were abolished by addition of the uncoupler. From these results it is concluded that energy coupling between the electron transfer from H₂ to the heterodisulfide and ATP synthesis occurs via a transmembrane proton gradient.

【 授权许可】

Unknown