Cloning and expression of a cDNA encoding a novel human neurotrophic factor

FEBS Letters

Nakahama, Kazuo¹  Kaisho, Yoshihiko¹  Yoshimura, Koji¹ 

[1] Biotechnology Research Laboratories, Takeda Chemical Industries Ltd, 17-85 Jusohonmachi 2-chome, Yodogawa-ku, Osaka 532, Japan

关键词: Novel neurotrophic factor;    cDNA cloning;    Gene family;    Glioma cell;   

DOI  :  10.1016/0014-5793(90)81536-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a novel human neurotrophic factor (designated nerve growth factor-2; NGF-2) was cloned from a human glioma cDNA library using a synthetic DNA corresponding to human nerve growth factor (NGF). The cloned cDNA encodes a polypeptide composed of 257 amino acid residues including a prepro-sequence of 138 residues and a mature region of 119 residues. The amino acid sequence of human NGF-2 exhibits 58% similarity with that of human NGF. Conditioned medium of COS-7 cells transfected with an expression plasmid for human NGF-2 cDNA supported the survival of sensory neurons isolated from dorsal root ganglia of embryonic chicks. A 1.5 kb of NGF-2 mRNA can be detected from an early development stage in rat brain, by Northern blotting analysis.

【 授权许可】

Unknown